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J Exp Bot 67:6037–6049Ĭhen ZX, Li FL, Yang SN, Dong YB, Yuan QH, Wang F et al (2013) Identification and functional analysis of flowering related microRNAs in common wild rice ( Oryza rufipogon Griff.). Genome Biol 12:R53įei Q, Yang L, Liang W, Zhang D, Meyers BC (2016) Dynamic changes of small RNAs in rice spikelet development reveal specialized reproductive phasiRNA pathways. Wei LQ, Yan LF, Wang T (2011) Deep sequencing on genome-wide scale reveals the unique composition and expression patterns of microRNAs in developing pollen of Oryza sativa. Millar AA, Gubler F (2005) The Arabidopsis GAMYB-like genes, MYB33 and MYB65, are microRNA-regulated genes that redundantly facilitate anther development. PLoS Biol 9:e1001155īencivenga S, Colombo L, Masiero S (2011) Cross talk between the sporophyte and the megagametophyte during ovule development. Schmidt A, Wuest SE, Vijverberg K, Baroux C, Kleen D, Grossniklaus U (2011) Transcriptome analysis of the Arabidopsis megaspore mother cell uncovers the importance of RNA helicases for plant germline development. Panoli AP, Ravi M, Sebastian J, Nishal B, Reddy TV, Marimuthu MPA et al (2006) AtMND1 is required for hoMogous pairing during meiosis in Arabidopsis. Development 127:197–207īarrell PJ, Grossniklaus U (2005) Confocal microscopy of whole ovules for analysis of reproductive development: the elongate1 mutant affects meiosis II. Siddiqi I, Ganesh G, Grossniklaus U, Subbiah V (2000) The dyad gene is required for progression through female meiosis in Arabidopsis. Yang WC, Shi DQ, Chen YH (2010) Female gametophyte development in flowering plants. These data may provide important clues for further revealing the mechanism of female meiosis in rice. Through bioinformatics analysis, we obtained many differentially expressed genes, miRNAs, and differentially methylated genes related to female meiosis.

In order to systematically study the potential mechanism of gene expression and regulation in female meiosis, ovules at megaspore mother cell meiosis stage, functional megaspore mitosis stage, and mature female gametophytes are collected to perform genome-wide RNA sequencing, small RNA sequencing, and bisulfite sequencing. As important gene regulatory factors, miRNAs and DNA methylation are widely involved in plant meiosis and ovule development. However, the mechanism of gene expression and regulation in female meiosis process is still poorly understood. In rice ovule, megaspore mother cells undergo meiosis to form megaspores then the functional megaspore performs three rounds of mitoses to form female gametophyte. Meiosis is an essential process in sexual life cycle, not only for the genomic stability maintenance but also for the genetic diversity creation through recombination.

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